Among these cards are 389 Major Leaguers and 134 Minor Leaguers. Taking into account the possibilities of over 520 different player poses on the fronts, and 16 different advertisement backs, there are thousands of permutations of cards to complete a "set".  There are also multiple cards for the same player in different poses, different uniforms, or even with different teams after being traded (since the set was issued over a period of several years). The cards measure 1-7/16" x 2-5/8" which is considered by many collectors to be the standard tobacco card size.

The card fronts feature a color lithograph of a player surrounded by a white border. A few cards were printed in a horizontal format, but almost all of the 523 cards in the set were oriented vertically. Card backs do not contain any statistics; instead, an advertisement appears for the cigarette brand the card was packaged with. The cards were printed on sheets by one factory, and each brand was allowed to place its ad on the backs.  Some of the card backs include: Piedmont, Tolstoi, Sweet Caporal, Cycle, Drum, Old Mill, Polar Bear, Sovereign, Hindu, Lenox, Uzit, El Principe, and even a Ty Cobb version.  Additionally, some blank-backed cards have been found.

The Honus Wagner card is the most rare of all.  It is estimated that between 50 and 200 of the Wagner cards were ever distributed to the public, and fewer still have survived to the present day. Several theories exist as to why the card is so scarce. One theory is that the printing plate used to create Wagner's card broke early in the production process. Another theory is that there was a copyright dispute between the American Tobacco Company and the artist who created the Wagner lithograph which resulted in a reduced production.

The most commonly accepted theory is that the card was pulled from production because Wagner himself objected to the production of the card, but his motivation is unclear. Reports at the time indicated that Wagner did not wish to associate himself with cigarettes, possibly because he did not want to encourage children to smoke.  However, some collectors and historians have pointed out that Wagner, a user of chewing tobacco, allowed his image to appear on cigar boxes and other tobacco-related products prior to 1909 and objected to the card simply because he wanted more financial compensation for the use of his image.